Disposable Microchamber with a Microfluidic Paper-Based Lid for Generation and Membrane Separation of SO2 Gas Employing an In Situ Electrochemical Gas Sensor for Quantifying Sulfite in Wine.

This work presents a fully disposable microchamber for gas generation of a sample solution. The microchamber consists of a cylindrical well-reactor and a paper-based microfluidic lid (µFluidic lid), which also serves as the reagent loading and dispensing unit. The base of the reactor consists of a hydrophobic membrane covering an in-house graphene electrochemical gas sensor. Fabrication of the gas sensor and the three-layer µFluidic lid is described. The µFluidic lid is designed to provide a steady addition of the acid reagent into the sample solution instead of liquid drops from a disposable syringe. There are three steps in the procedure: (i) acidification of the sample in the reactor to generate SO2 gas by the slow dispensing of the acid reagent from the µFluidic lid, (ii) diffusion of the liberated SO2 gas through the hydrophobic membrane at the base of the reactor, and (iii) in situ detection of SO2 by cathodic reduction at the graphene electrode. The device was demonstrated for quantitation of the sulfite preservative in wine without heating or stirring. The selectivity of the analysis is ensured by the combination of the gas-diffusion membrane and the selectivity of the electrochemical sensor. The linear working range is 2-60 mg L-1 SO2, with a limit of detection (3SD of intercept/slope) of 1.5 mg L-1 SO2. This in situ method has the shortest analysis time (8 min per sample) among all voltammetric methods that detect SO2(g) via membrane gas diffusion.

Simple gunshot residue analyses for estimating firing distance: Investigation with four types of fabrics.

This work presents two simple methods for estimating the firing distance from the gunshot residues (GSRs) on fabric targets. Four types of fabric targets, namely twill weave denim cotton-polyester (80/20), jersey knitting 100% cotton, plain weave cotton-polyester (80/20) and plain weave cotton-polyester (60/40), were employed. The firing tests were carried out using these white fabrics as targets at distances of 5-100 cm, respectively. In the first method, digital images of the black GSRs on fabric materials were recorded inside an illuminated box and the inverted gray intensity values were plotted against the firing distances. Since the plots of all fabrics are not significantly different, the estimation of firing distance employs the same exponential curve for all test fabrics. Although simple, the imaging method is not suitable for dark-colored materials. A chemical-based method was therefore developed as an alternative method. In the second method, a small disposable microfluidic paper-based analytical device (µPAD) was employed for detecting Pb(II) extracted from the GSRs. The µPAD method uses the measurement of the length of a narrow band of a pink color resulting from reaction between rhodizonate reagent and the Pb(II) extract. The plots indicated that the data of thick denim material are significantly different to other test fabrics which are much thinner. These three fabrics share the same estimation curve. However, it is recommended that the separate estimation curve for denim materials must be used. Both methods are suitable for short range firing distance, no further than 60 cm, since at greater distances the inverted gray intensity and the 'band-length' methods are unable to detect the GSRs.

Gold leaf electrochemical sensors: applications and nanostructure modification.

This work presents the first planar three-electrode electrochemical sensor comprising local gold leaf as the working electrode and printed, or hand-drawn, counter and reference electrodes, respectively. The gold leaf was mounted on a polyvinyl chloride (PVC) adhesive sheet (15 mm × 30 mm) and covered with a second PVC sheet printed with the counter and reference electrodes. This sheet has a 3 mm circle and a 2 mm × 3 mm rectangle removed to expose the gold electrode area and electrical contacts, respectively. A third shorter insulating layer with a 10 mm circular hole was placed on top to delineate the sensing area of all electrodes. The sensor displayed expected performances in various modes of operation, such as cyclic voltammetry, square wave voltammetry and anodic stripping voltammetry. For the latter mode, the limit of detection of Pb(ii) was 3.2 µg L-1, compliant with regulation for drinking water (10 µg L-1 Pb(ii)). Although designed as a disposable unit, the electrode is effective for up to 200 cycles and applicable for multiple use. The gold leaf was modified by electrodeposition of the gold network and large nano-size gold particles which significantly enhanced the sensitivity of all voltametric sensing, giving lower limits of detection. For stripping voltammetry, the electroplating structure modification improved the simultaneous detection of lead and copper, with the copper response increasing 6-fold. The device has the capability of on-site identification of copper/lead bullets from gunshot residues within 6 min.

T-shirt ink for one-step screen-printing of hydrophobic barriers for 2D- and 3D-microfluidic paper-based analytical devices.

This work presents the use of polyvinyl chloride (PVC) fabric ink, commonly employed for screening t-shirts, as new and versatile material for printing hydrophobic barrier on paper substrate for microfluidic paper-based analytical devices (µPADs). Low-cost, screen-printing apparatus (e.g., screen mesh, squeegee, and printing table) and materials (e.g. PVC ink and solvent) were employed to print the PVC ink solution onto Whatman filter paper No. 4. This provides a one-step strategy to print flow barriers without the need of further processing except evaporation for 3-5 min in a fume hood to remove the solvent. The production of the single layer µPADs is reasonably high with up to 77 devices per screening with 100% success rate. This method produces very narrow fluidic channel 486 ±â€¯14 µm in width and hydrophobic barrier of 642 ±â€¯25 µm thickness. Reproducibility of the production of fluidic channels and zones is satisfactory with RSDs of 2.9% (for 486-µm channel, n = 10), 3.7% (for 2-mm channel, n = 50) and 1.5% (for 6-mm diameter circular zone, n = 80). A design of a 2D-µPAD produced by this method was employed for the colorimetric dual-measurements of thiocyanate and nitrite in saliva. A 3D-µPADs with multiple layers of ink-screened paper was designed and constructed to demonstrate the method's versatility. These 3D-µPADs were designed for gas-liquid separation with in-situ colorimetric detection of ethanol vapor on the µPADs. The 3D-µPADs were applied for direct quantification of ethanol in beverages and highly colored pharmaceutical products. The printed barrier was resistant up to 8% (v/v) ethanol without liquid creeping out of the barrier.

Membraneless Gas-Separation Microfluidic Paper-Based Analytical Devices for Direct Quantitation of Volatile and Nonvolatile Compounds.

This work presents new chemical sensing devices called "membraneless gas-separation microfluidic paper-based analytical devices" (MBL-GS µPADs). MBL-GS µPADs were designed to make fabrication of the devices simple and user-friendly. MBL-GS µPADs offer direct quantitative analysis of volatile and nonvolatile compounds. Porous hydrophobic membrane is not needed for gas-separation, which makes fabrication of the device simple, rapid and low-cost. A MBL-GS µPAD consists of three layers: "donor layer", "spacer layer", and "acceptor layer". The donor and acceptor layers are made of filter paper with a printed pattern. The donor and acceptor layers are mounted together with a spacer layer in between. This spacer is a two-sided mounting tape, 0.8 mm thick, with a small disc cut out for the gas from the donor zone to diffuse to the acceptor zone. Photographic image of the color that is formed by the reagent in the acceptor layer is analyzed using the ImageJ program for quantitation. Proof of concept of the MBL-GS µPADs was demonstrated by analyzing standard solutions of ethanol, sulfide, and ammonium. Optimization of the MBL-GS µPADs was carried out for direct determination of ammonium in wastewaters and fertilizers to demonstrate the applicability of the system to real samples.